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Simple, Field-Usable Fluorescence-Based Isothermal LAMP Assay for the On-Site Diagnosis of Malaria
CDC researchers have developed improved Loop-Mediated Isothermal Amplification (LAMP) assays for the nucleic acid-based diagnosis of malaria in field settings. The approach employs Plasmodium genus-specific LAMP primers and a portable tube scanner to run the LAMP reaction and measure fluorescence signal (e.g., SYBR green) as a measure of DNA amplification...
Published: 5/9/2025   |   Inventor(s): Allison Demas, Naomi Lucchi, Venkatachalam Udhayakumar
Keywords(s): assay, Based, CDC Docket Import, CDC Docket Import CDC Prosecuting, CDCRM, DA2XXX, DAXXXX, DDXXXX, DEVELOPING, Development, Diagnosis, diagnostic, DXXXXX, Field-usable, Fluorescence, Isothermal, ivd, LAMP, LIMITED-RESOURCE, LOW INPUT, Malaria, Mosquito, Mosquitoes, nucleic acid, NUCLEIC ACID DETECTION, PCR ALTERNATIVE, real time, REAL-TIME, SIMPLE, travel, traveler, travelers, VBXXXX, Vector-borne, Vector-borne diseases, VJXXXX, VKXXXX, VLXXXX, VOXXXX, WAXXXX, WBXXXX, WDXXXX, WFXXXX, WIXXXX, WMXXXX, YBXXXX, Zoonotic
Category(s): Collaboration Sought > Licensing, Application > Diagnostics, Application > Research Materials, Application > Occupational Safety and Health, Application > Therapeutics, Application > Vaccines, TherapeuticArea > Infectious Disease, Application > Consumer Products
Real-time RT-PCR Assay For Detection and Quantification of Hepatitis D Virus Infection
CDC scientists have developed a one-step TaqMan quantitative/real-time reverse transcription-polymerase chain reaction (qRT-PCR) assay for detecting hepatitis D virus (HDV) RNA. Additionally, a quantifiable synthetic RNA control to determine viral load has been created. HDV is an operatively defective virus that requires hepatitis B virus (HBV) surface...
Published: 5/9/2025   |   Inventor(s): Tonya Mixson-Hayden, Saleem Kamili, Maja Kodani
Keywords(s): assay, CDC Docket Import, CDC Docket Import CDC Prosecuting, cirrhosis, D, DA4BXX, DA4XXX, DAXXXX, Detection, diagnostic, Diagnostic assay, DXXXXX, HBV, HDV, Hepatitis, HEPATITIS B, liver, liver disease, Liver disorders, Liver-Disease, OID-NCHHSTP-DVH, PCR, QUANTITATIVE, REAL, real time, REAL-TIME, RT-PCR, viral, virus
Category(s): Collaboration Sought > Licensing, Application > Diagnostics, TherapeuticArea > Infectious Disease