Search Results - mattheos+koffas

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Recombinant Enzyme for the Accurate Insertion of Novel DNA Sequences in Eukaryotic Cells
RPI ID: 2022-044-301 Innovation Summary: This gene editing system introduces a recombinant enzyme composed of an HIV integrase (HIV IN) tetramer and two or more Transcription Activator-Like Effectors (TALEs) bound via polypeptide linkers. The enzyme is designed to enable site-specific DNA insertion in eukaryotic cells with minimal off-target effects....
Published: 3/10/2026   |   Inventor(s): Mattheos Koffas, William Lawler
Keywords(s): Gene Integration, Genetic Engineering, Recombinant Proteins, Somatic Cell Editing
Category(s): Biotechnology and the Life Sciences
Downregulation of Promoter Strength for Increased Production of Recombinant Biopolymers
RPI ID: 2021-070-401 Innovation Summary: Recombinant E. coli strains and synthetic protein sequence designs are used to produce disordered polypeptides such as spidroins and elastin-like peptides (ELPs). The system leverages genome engineering to suppress basal expression and redirect cellular resources toward target biopolymer synthesis. This approach...
Published: 3/10/2026   |   Inventor(s): Mattheos Koffas, Derek Nelson, Runye Zha, Alexander Connor
Keywords(s): Biomanufacturing, Biomaterials, Recombinant Protein Engineering, Sustainable Materials
Category(s): Biotechnology and the Life Sciences
Upcycling of Plastic Waste to Recombinant Silk Proteins via Pseudomonas sp
RPI ID: 2021-045-401 Innovation Summary: This method uses engineered Pseudomonas bacteria to convert plastic waste into recombinant silk proteins. The system includes metabolic pathways that degrade polyethylene terephthalate (PET) and redirect carbon into silk protein synthesis. It supports circular bioeconomy models and reduces environmental impact....
Published: 3/10/2026   |   Inventor(s): Runye Zha, Mattheos Koffas, Alexander Connor
Keywords(s): Advanced Materials, Biomanufacturing, Metabolic Engineering, polymer upcycling, sustainability
Category(s): Biotechnology and the Life Sciences
Microbial Synthesis of Sulfated Glycosaminoglycans
RPI ID: 2020-014-401 Innovation Summary: Recombinant microorganisms are engineered to biosynthesize sulfated glycosaminoglycans (GAGs) directly in vivo, bypassing the need for animal-derived sources. The system incorporates genetic pathways for polymerization and sulfation, enabling controlled production of biologically active GAGs. This approach supports...
Published: 3/10/2026   |   Inventor(s): Abinaya Badri, Mattheos Koffas, Robert Linhardt, Asher Williams
Keywords(s): Animal-free Glycosaminoglycans, Metabolic engineering of E. coli, One pot microbial production of sulfated biologics
Category(s): Biotechnology and the Life Sciences
Recombinant Production of Silk Proteins via Bacillus Megaterium Secretion (Updated)
RPI ID: 2023-070-301 Innovation Summary: This technology enables the recombinant production of silk proteins using Bacillus megaterium as a secretion host. The system leverages the bacterium’s natural secretion pathways to produce and release silk proteins extracellularly, simplifying downstream purification. The method supports scalable production...
Published: 3/10/2026   |   Inventor(s): Mattheos Koffas, Runye Zha, Alexander Connor
Keywords(s): advanced polymers, Biodegradable materials, Biomaterial, Biomedical material, Biomimetic material, green polymer synthesis, Materials, silk, sustainable
Category(s): Biotechnology and the Life Sciences