High-Throughput DNA Origami Nanoarrays

Traditional bulk diagnostic assays suffer from signal dilution and averaging, making it difficult to detect rare biomarkers and subtle molecular variations critical for early disease diagnosis. Microarrays address this limitation by enabling high-throughput, multiplexed analysis with improved specificity and sensitivity. Their structured array format allowing simultaneous detection of multiple targets to provide a more accurate and comprehensive molecular profile compared to bulk approaches.

The integration of single-molecule detection with microarrays further enhances their diagnostic potential by pushing sensitivity to the absolute molecular level, allowing quantification of low-abundant biomarkers. This synergy enables the early detection of rare disease biomarkers that would otherwise be undetectable in bulk approaches, refining precision medicine. By combining the high-throughput capabilities of microarrays with the resolution of single-molecule analysis, researchers and clinicians can achieve unprecedented accuracy in biomarker discovery, treatment monitoring, and disease characterization.

Researchers at the Biodesign Institute of Arizona State University have developed a novel method using nanosphere lithography to create high-density, periodic DNA origami nanoarrays. This technology enables precise single-molecule placement of DNA origami in a microarray, allowing for targeted modifications to form functional nodes. A hydrophobic barrier around each node enhances single-origami occupancy, increasing active sites for downstream processes. This cleanroom-free fabrication approach lowers chip production costs while enabling high-throughput, precise molecule positioning. This advancement paves the way for next-generation diagnostics that are highly sensitive, specific, and scalable with far-reaching economic implications.

These DNA origami nanoarrays and cleanroom-free, bottom-up fabrication approach have the potential to revolutionize nanoscale fabrication with far-reaching economic implications.

Potential Applications

Biophysical assays and diagnostics tools
Biosensing and molecular electronics
Drug discovery

Benefits and Advantages

Enables high-density, periodic DNA origami nanoarrays on glass surfaces
Reduces sticky patch size to less than 100 nm, minimizing multiple origami occupancy and maximizing number of binding sites
Maintains the periodicity of the nanoarray and allows for the creation of distinct, well-separated features that can be well resolved
Enhances reliable and accurate data collection
Cleanroom-free fabrication reduces cost-per-chip compared to top-down fabrication methods
More accessible and cost-effective for researchers and industry professionals
The formation of the hydrophobic barrier enables this to be compatible with circular DNA origami structures
Facilitates high-throughput, single-molecule experiments
Ability to customize the nanoarrays for specific applications makes this versatile and adaptable

For more information about the inventor(s) and their research, please see

Dr. Hariadi's departmental webpage

Dr. Hariadi’s laboratory webpage