This innovative technology addresses a significant challenge in lipid analysis, setting it apart from conventional methods. By utilizing high-frequency surface acoustic waves (SAW) for mechanical disruption of lipid assemblies like liposomes and extracellular vehicles (EVs), it eliminates the need for chemical treatments that may alter the sample composition. This disruption approach offers a direct pathway to analyze constituent lipids, including nonpolar lipids like cholesterol, while maintaining the integrity of the vesicles. Additionally, the incorporation of SAW nebulization with corona discharge (CD) ionization enables the direct mass spectrometry (MS) analysis of liposome-derived lipids and other molecules in aqueous samples, expanding the applicability to native MS conditions. This novel technology not only improves the accuracy of lipid analysis but also enhances the potential for advancements in drug discovery, delivery, and broader lipid-related research. Its non-destructive, precise, and versatile approach makes it a game-changer in the field of lipid assembly analysis and MS-based research.
What sets this technology apart is its unique approach to lipid assembly analysis. Unlike traditional methods that rely on chemical disruption, it harnesses the power of high frequency surface acoustic waves (SAW) to mechanically disrupt lipid structures, preserving their integrity and composition. This innovative SAW-based approach allows for direct mass spectrometry (MS) analysis of lipids, including nonpolar lipids like cholesterol, in a native state, without the need for chemical modifications. The incorporation of SAW nebulization with corona discharge (CD) ionization further enhances its versatility, making it applicable to aqueous samples. This technology offers a non-destructive, precise, and efficient solution to the challenges of lipid analysis, making it a groundbreaking advancement in the field.
Stage 1: SAW lysing and stage 2: SAW Nebulization and Ionization.