NU 2018-182
INVENTORS
Yevgenia Kozorovitskiy*
Manish Kumar
SHORT DESCRIPTION
A novel oblique plane imaging system utilizing a reduced number of objectives to enable the use of low numerical aperture (NA) objectives, thus achieving a compact and cost-effective design while maintaining high-quality imaging performance.
BACKGROUND
Traditional oblique plane imaging systems employ three high NA objectives arranged sequentially, which result in a significant reduction of the system's effective NA. This configuration limits the use of these systems to high NA objectives, increasing complexity and cost. Addressing these challenges, this invention proposes alternative arrangements that simplify the system and allow for the use of low NA objectives without sacrificing imaging quality.
ABSTRACT
This innovative imaging system offers two solutions to overcome the limitations of conventional oblique plane microscopy. The first solution involves removing the third objective and positioning the image sensor directly at the intermediate image plane. The second solution uses a diffusive screen at this plane, followed by a magnifying system. Both approaches enable the use of low NA objectives, leading to a smaller, more affordable system while preserving the benefits of oblique plane imaging, such as steric access and 3D reconstruction capabilities.
APPLICATIONS
Structural and functional imaging
For optically scattering fluorescent brain samples.
In vivo fast volumetric imaging
Suitable for zebrafish imaging.
Neuronal activity studies
Using genetically encoded calcium indicators in large 3D volumes.
Portable imaging systems
Capable of high-rate 3D volumetric imaging.
ADVANTAGES
Simplified geometry
Enables high-resolution imaging with low NA objectives.
Compact design
Allows for system miniaturization.
Constant tilt scanning
Provides distortion-free 3D reconstruction.
Cost-effective
Substantially reduces system price.
Steric access
Maintains steric accessibility to samples.
PUBLICATIONS
Manish Kumar, Yevgenia Kozorovitskiy et al, "Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging", Optics Express, Vol. 26, Issue 10, pp. 13027-13041 (2018).
IP STATUS
Issued US Patent 12,105,270