Case 2014-180
UCLA researchers have developed a novel method of detecting molecules of interest which can be performed without washing steps or any solid-phase reaction.
BACKGROUND
The typical sandwich ELISA utilizes adsorbed antibodies on a surface that capture antigens and a secondary antibody with sequential washes to provide a detection signal. Others have used antibody-coated beads coupled with femtoliter-sized wells to fractionate the sample and generate a potentially more robust assay with a digital output. However, since these assays require one bead per well, the bead capture efficiency limits the sensitivity of the assay. Additionally, both traditional and digital ELISAs require sequential addition of reagents for binding as well as a number of wash steps to remove unbound reagents. Existing assays require detection antibodies to be immobilized on a surface, including bead-based assays.
INNOVATION
Researchers at UCLA have identified a novel method of detecting molecules of interest which can be performed without washing steps or any solid-phase reaction. Since this assay does not require a surface or beads to adsorb to and other sample preparation steps, this assay is ideally suited for point-of-care use. Instead, this assay requires specially modified antibodies to allow for amplification of the detection event. This assay does not require a number of wash steps or sequential addition of reagents, which is typical of current assays; instead this assay just requires a single mix step of the reagents with the target sample.
APPLICATIONS
ADVANTAGES
STATE OF DEVELOPMENT
Various aspects of the assay have been developed, including the microfluidics aspect that is important to enable the homogenous assay. Testing of the reaction components in a well plate as well as within the device has been carried out, but not optimized.