Regenerative medicine urgently needs standardized methods to manufacture therapeutic secretomes secreted by corneal stromal stem cells, yet current culture systems fall short. Traditional two-dimensional and static three-dimensional platforms cannot replicate natural physiological conditions, causing nutrient gradients, hypoxic cores, and cellular stress. These conditions reduce stem cell quality and stemness, producing inconsistent and low-efficacy secretomes and creating serious manufacturing bottlenecks that block clinical translation.
This modular 3D dynamic culture platform uses a cross-linked sodium alginate and collagen scaffold paired with a printed 3D holder and syringe pump that delivers controlled bidirectional media flow. The biomimetic scaffold offers a near neutral surface charge, visco elastic properties, and a porous structure that securely holds stem cells for their healthy growth and secretion of secretomes. This carefully controlled environment sustains high cell viability and preserves key stem cell markers, enabling standardized, reproducible production of stem cells and their therapeutic secretomes for consistent, scalable manufacturing.