Search Results - tyler+dangerfield

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Modified CRISPR-Cas9 system for enhanced gene editing
This invention enhances the CRISPR-Cas9 gene-editing system by modifying the Cas9 protein’s Rec3 clamp region, improving its fidelity, specificity, and speed, reducing off-target effects, and increasing precision for therapeutic and research applications. Background Genome engineering is a rapidly advancing field that involves the precise modification...
Published: 10/21/2024   |   Inventor(s): David Taylor, Kenneth Johnson, Jack Bravo, Tyler Dangerfield
Keywords(s):  
Category(s): Life sciences > Research tools > Reagent
SuperFi high-fidelity Cas9 enzyme eliminates off‑target CRISPR cleavage
Background The widespread use of CRISPR/Cas9 as a programmable genome editing tool has encountered hurdles, due to concerns about potential off‑target DNA cleavage by Cas9. While the analysis of off-target editing events has led to the develop­ment of Cas9 variants with greater sensitivity to mismatches, the underlying molecular mechanisms by which...
Published: 2/29/2024   |   Inventor(s): David Taylor, Kenneth Johnson, Jack Bravo, Mu-Sen Liu, Tyler Dangerfield
Keywords(s):  
Category(s): Life sciences > Research tools > Reagent