Label-free Fluorescence-based Biopolymer Sequencing using Nanopore Arrays

INV-14062

 

Nanopore sequencing is a third generation approach used in the sequencing of biopolymers, where polynucleotides are in the form of DNA or RNA. Using nanopore sequencing, a single molecule of DNA or RNA can be sequenced without the need for PCR amplification or chemical labeling of the samples. 

Nanopore sequencing has the potential to offer relatively low-cost genotyping, high mobility for testing, and rapid processing of samples with the ability to display results in real-time.

 

This technology provides a novel approach for high throughput Nanopore-Based DNA sequencing. The system uses an ion sensitive florescent reporter molecule, which provides optical detection of changes in the ion current when analyte molecules enter into the nanopore arrays. When an analyte molecule enters a pore, it obstructs the ionic current, resulting in a change in fluorescence. 

 

- Enables simultaneous reading of large nanopore arrays (others only read from a single nanopore)

- Is label-free

- Can be controlled with subnanometer precision, opening up a whole range of molecules 

 

- DNA sequencing

- RNA sequencing

- Protein sequencing

 

- License

- Partnering

- Research collaboration