Human Lamin Expression Vectors

NU 1997-011

Inventors

Robert Goldman*

Robert Moir

Short Description

Protein expression vectors for all three human nuclear lamin proteins

Abstract

Northwestern scientists have developed protein expression vectors for recombinant production of nuclear lamins in Escherichia coli. There are at least 3 lamin proteins in human cells and together they accomplish several functions important for nuclear integrity, nuclear import and DNA replication. Because of these important functions, lamins have been extensively studied. These overexpression plasmids will facilitate research of lamins and enable generation of other research reagents as well.

Applications

• Research tool for study of nuclear lamin proteins
• Production of nuclear lamins

Advantages

• Simple
• Efficient

Publications

Moir, RD, Lowy-Montag M, Goldman RD (1994) Dynamic Properties of Nuclear Lamins: Lamin B is Associated with Sites of DNA Replication. Journal of Cell Biology. 125: 1201-1212.