Brief Description: A lung-on-a-chip microfluidic chip, comprising: a first region, the first region comprising a central channel and at least one side channel adjacent thereto, the central channel having therein a plurality of cells disposed in a matrix; and a second region, the second region comprising a vacuum chamber; and a membrane disposed between the central channel and the vacuum chamber, the membrane arranged so as to maintain fluidic isolation between the central channel and the vacuum chamber, and the membrane arranged such that a pressure within the vacuum chamber effects movement of the membrane such that the plurality of cells disposed in the matrix experience a mechanical force related to the movement of the membrane. A method, comprising changing a pressure within the vacuum chamber of a microfluidic chip according to the present disclosure. Image: Caption: Reference Media Title: Reference Media: Intellectual Property Title: Intellectual Property: Intellectual Property: US 2025/0115837 A1 Docket Number: Docket#: 24-10579