Search Results - james+rybarski

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Bioinformatics-powered CRISPR-transposon systems for enhanced gene editing and horizontal gene transfer
CRISPR-associated transposons (CASTs) use nuclease-deficient CRISPR-Cas systems for RNA-guided gene insertion, enhancing the gene-editing toolkit by enabling precise DNA integration. Background CRISPR-associated transposons (CASTs) represent a significant advancement in gene-editing technology, originating from the fusion of CRISPR-Cas systems with...
Published: 8/8/2024   |   Inventor(s): Ilya Finkelstein, James Rybarski, Kuang Hu, Alexis Hill, Claus Wilke
Keywords(s):  
Category(s): Life sciences > Research tools > Reagent
Predicting off-target activity of CRISPR-Cas9 complexes
Problem CRISPR-Cas9 is originally a bacterial defense system that uses a guide-RNA to target and nucleases to cut foreign nucleic acids. Recently, these protein-RNA complexes have taken the genome editing world by storm since the revolutionary discovery was made that they could be used as genetic editing tools. By reprogramming the guide-RNA, the Cas9...
Published: 11/1/2023   |   Inventor(s): Ilya Finkelstein, Cheulhee Jung, John Hawkins, Stephen Jones, James Rybarski, Mashelfatema Saifuddin, Cagri Savran, Andrew Ellington, William Press
Keywords(s):  
Category(s): Life sciences > Research tools > Tool/assay/screening platform