Kinetic chromogenic (CG) and fluorogenic (FG) quantification deduces analyte concentration based on the reaction rate between the CG/FG probe and its targeted molecule. Little progress has been made in the past half century in either the theory or the applications of the kinetic spectroscopic quantification methods. Current kinetic CG/FG quantification is limited only to a subset of CG/FG reactions that can be approximated as the single-step process, and more problematically, to research samples with no matrix interferences.
This invention relates to a comprehensive system compromising devices, sampling accessories, and reagents designed for the swift kinetic fluorogenic quantification of chemicals. It enables the quantification of malondialdehyde (MDA), a widely utilized lipid peroxidation biomarker in the food industry and biological research. Moreover, this technology can be readily adapted to encompass a wide spectrum of biomarkers and chemical agents, showcasing its versatility and broad applicability.
This technology has diverse applications in biological, clinical, environmental, and chemical contexts, as well as detection and quantification of chemical warfare agents on battlefields. It could also be utilized for efficient process monitoring and quality control in industries including food, biomanufacturing, and beverage production.