Transcriptome In Vivo Analysis (TIVA)

Technology Overview: 
Transcriptome in vivo analysis (TIVA) is a novel platform that enables spatial and temporal analysis of the transcriptome in single or multiple cells from a heterogeneous population. TIVA can be combined with numerous in vivo and in vitro functional analysis techniques which are not supported by current RNA isolation methodologies.

Applications: 

• Temporal RNA transcriptome analysis within a cell
• Spatial RNA analysis within a cell (e.g. dendrites of neurons or apical region of a stromal cell)
• Rapid RNA capture from individual cells within the same tissue
• Analysis of RNA in cells that are connected or in communication with one another
• Measurement of in vivo rates of RNA accumulation or degradation
• Use with other in vivo techniques, such as live-imaging or electrophysiology. 

Intellectual Property: 

• US 9,540,634

Reference Media: 

• Lovatt, D. et al.;  Nat Methods. 2014 Feb; 11(2):190

Docket # W5534