This invention is a new tool and technique for identification of antigen-specific t cells. By using specific molecules from specified cell lines as antigen presenting cells, the identified T cells are labeled with different fluorescence. These cells go through the flow cytometry and the flow cytometry data is analyzed by a specific assay designed by the inventor to identify the antigen-specific T cells. The new tool and approach can detect these antigen specific T-cells with TCR affinity lower than closest known products. Background: T cells are essential for long-lived, protective immunity against microbial infections and other diseases such as cancer. T cell detection and monitoring technology is critical to advance understanding of adaptive immunity for patients with microbial diseases or complex diseases such as cancer. In addition to monitoring a patient’s immune system, these cells can evaluate the efficacy of vaccinations and other therapeutics against diseases. Current solutions and systems for detection and monitoring of T cells, fail to detect 50-95% of pMHC-specific T cells. Applications:
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