Real-Time multiplex Measurement of Antibody-Antigen Binding Constant using Lab-on-a-Chip (LOC) device

INV-14118

 

A major challenge in the detection of soluble molecules, such as biomolecules, drugs, proteins, antigens, and antibodies, is the ability to monitor time‑varying concentrations and reaction association/dissociation constraints in real‑time. 

This technology comprises a microsphere-based immunoassay fitted to a microfluidic-based format in order to develop a LOC (Lab‑On‑a‑Chip) device that integrates laboratory functions such as real-time monitoring of a target with clinically relevant analytes via antibody-antigen recognition effect. 

Further, a mathematical model has been developed to support the LOC real-time detection protocol and demonstrates the calculation of online and real‑time binding constants in the antibody‑antigen.

 

This technology uses a flow‑through microsphere‑based immunoassay in a microfluidic device. The polydimethylsiloxane (PDMS) based microfluidic system was designed and fabricated using standard soft lithography methods. 

This device consists of 3 inlets, 2 mixing regions, and a detector region. As the microsphere‑antibody and antigen solution flows through the serpentine channel and diffuses, the antigens are captured by the antibody‑coated microspheres. A secondary antibody against the antigen is then conjugated with a specific fluorophore.

This novel design of LOC can detect analytes and measure the binding constant of the reaction in real time by allowing the integration of bead-based assays and generating a well-mixed solution.

 

- Assay for real-time monitoring of multiple analytes

- Online‑binding constant beads can be used with a combination of microfluidic devices to detect trace amounts of analytes in an automated manner

- Real‑time multiplex of disease markers

- Real‑time multiplex of the pharmacokinetics of drugs

 

- Pharmacokinetic analysis in real‑time

- Detection of analytes in real time in‑vivo

- Antibody affinity demonstration in real‑time

 

- License

- Partnering

- Research Collaboration