Co-transactionally folded RNA nanostructures in living cells.
Invention Summary:
Biomarker validation and intracellular RNA detection require tools that are precise, scalable, and capable of capturing complex gene expression patterns. Current approaches, including CRISPR-based systems, RNA interference, and RNA sponge technologies, are often limited by a single-target focus, reliance on protein components, off-target effects, or a lack of structural organization. These limitations restrict their ability to perform multiplex RNA sensing and pathway-level analysis in living cells, which is increasingly needed in diagnostics, drug discovery, and functional genomics.
Rutgers researchers have developed a novel RNA nanostructure platform that self-assembles inside living human cells, enabling programmable and multiplex interactions with RNA and protein targets. Unlike existing RNA technologies, these co-transcriptionally folded RNA lattices form ordered, stable nanostructures that can integrate multiple functional modules, including sensing and binding domains, such as the KRAS proto-oncogene associated with pancreatic and colorectal cancers. This platform enables simultaneous detection and modulation of multiple intracellular targets, providing a new approach for studying complex biological pathways and developing next-generation diagnostic and therapeutic strategies.
Market Applications:
Advantages:
Publications:
Intellectual Property & Development Status: Provisional application filed. Patent pending. Available for licensing and/or research collaboration. For any business development and other collaborative partnerships, contact: marketingbd@research.rutgers.edu