Novel SDS-PAGE Sample Loading Buffer

SDS-PAGE is a ubiquitous analytical technique for separating proteins based on molecular weight. Existing loading buffers used in SDS-PAGE and other protein analyses techniques have difficulty in effectively solubilizing and stabilizing hydrophobic or lipidated proteins, particularly membrane proteins. These proteins tend to aggregate or precipitate in traditional buffers, which leads to incomplete denaturation and poor resolution and quantification.

 

Researchers at the Biodesign Institute of Arizona State University have developed a novel loading buffer composition and protocol for SDS-PAGE and Western Blotting. This buffer composition is formulated with more concentrated reagents to offer superior performance when running hydrophobic proteins on protein gels. When compared to standard loading buffers, this composition works just as effectively on SDS-PAGE, as shown using the highly sensitive silver staining method.

 

This buffer composition can overcome the challenges of traditional loading buffers and enhance the solubility and stability of hydrophobic and lipidated proteins to improve the accuracy and reliability of their analyses.

 

Potential Applications

Buffer for Western Blotting

For more information about the inventor(s) and their research, please see

Dr. Fromme’s laboratory webpage