Membrane-Based Devices for Multi-Step Assays
Need:
MicroPADs combine some of the capabilities of conventional microfluidic devices, such as the ability to perform multiple quantitative assays simultaneously, with the simplicity of later-flow immunoassays and dipstick assays. A significant challenge for microPADs is the ability to perform assays that require multiple, sequential steps. Ideally, a single sample-addition step is all that would be required to perform the assay. While, a third system for automating the delivery of reagents, erodible polymeric bridges, could be developed to perform multi-step assays from a single fluid-delivery step, but the valves are relatively complicated to fabricate.
Solution:
Researchers at Cal Poly have developed a new type of membrane-based fluidic device that is intended to be capable of performing multi-step assays from a single sample-addition step with no other input or actions required from the user. Membrane based devices for multi-step assays that include fluid storage zones intended to enable the automated sequential delivery of reagents, which are pre dried on the device, to a test zone preferably from a single addition of fluid on the device. These devices may be useful for performing multi-step assays automatically without requiring any input from the user after the initial sample is added to the device. In some aspects, multiple additions of fluids to the device may be used.
Applications:
- Enzyme inhibition assay
Advantages:
- Low cost
- Easier to use
- A single sample-addition step
IP Status:
US 10,279,344 issued 05/07/2019
Licensing Status:
Available - please contact us for more information.