PAGE TITLE
Overview
PAGE SUMMARY
Innovative methods for generating sequence-linked DNA fragments for high-quality, low-cost genome sequencing.
This technology describes a novel strategy to construct a long-read sequencing library such that adjacent fragments are linked with end-terminal duplications. The CRISPR-Cas9 nickase enzyme and a pool of multiple sgRNAs are used to perform non-random fragmentation of targeted long DNA molecules (>300kb) into smaller library-sized fragments (about 20 kbp) in a manner to retain physical linkage information (up to 1000 bp) between adjacent fragments. This sequencing strategy can be readily applied in both PacBio and Oxford Nanopore platforms and is useful for targeted enrichment of hundreds of target genomic regions and where long no-gap contigs need deep sequencing.
ADVANTAGES
TITLE:Key Advantages
Efficient generation of linked-paired end DNA fragments
Lower costs in assembling complex genomic regions
Improved throughput and accuracy of the sequencing
Problem Solved
TITLE:Problems Solved
Challenges in de novo genome assembly of sequence reads
Problems with mapping sequencing data to the reference sequence
Limitations in the resolution of haplotypes
Enrich the target genomic regions
APPLICATIONS
TITLE: Market Applications
Haplotype-resolved whole genome sequencing
High-throughput sequencing in genomics
Structural variant analysis
Enriched long-read target sequencing
IP STATUS
Intellectual Property and Development Status
United States Patent Pending- #US20240035024A1 - Linked-read sequencing library preparation
Canada Patent Pending - #CA3195700A1 - Linked-read sequencing library preparation
European Patent Pending - #EP4229220A1 - Linked-read sequencing library preparation
PUBLICATIONS
References
Pubinfo should be the citation for your publication. Publink is the full url linking to the publication online or a pdf.
Nature Scientific Reports 2024: A long-read sequencing strategy with overlapping linkers on adjacent fragments (OLAF-Seq) for targeted resequencing and enrichment
Commercialization Opportunities
This invention is part of a larger portfolio of technologies available for licensing and commercialization around TOOLS FOR GENOMIC APPLICATIONS POWERED BY CRISPR/CAS9 BASED DNA LABELING
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Contact Information
For Intellectual Property and Licensing inquiries
Tanvi Muni, PhD
Licensing Manager,
Drexel Applied Innovation
Office of Research and Innovation
3250 Chestnut Street, Ste. 3010 Philadelphia, PA 19104
Phone:267-359-5640
Email:tanvi.muni@drexel.edu
Inventor information
Ming Xiao, PhD
Professor School of Biomedical Engineering, Science and Health Systems
Phone:215-895-2690
Email:mx44@drexel.edu
Inventor Webpage