Adenosine diphosphate ribose (ADPR) is a biologically important molecule that is known to regulate oxidative stress, calcium homeostasis, and mitochondrial function. Very little is known about ADPR signaling because it is a fast and transient process. Our innovation addresses this challenge by streamlining the development of fluorescent biosensors, including for the critical NAD+ derived metabolite ADPR. By leveraging our platform, multiple sensor development efforts can be consolidated into one, expediting the process.
Grounded in evolutionary principles, our approach exploits conserved structural changes to develop genetically encoded sensors, specifically for molecules like adenosine diphosphate ribose (free ADPR). This methodology has already yielded success with sensors for three distinct molecules, with potential for further development of single fluorescent protein biosensors for various other molecules.
Our invention comprises a sensor that undergoes changes in fluorescence intensity upon binding to free ADPR. These sensors are genetically encoded, allowing for precise subcellular targeting and continuous monitoring of metabolic processes.
The University of Texas at Austin seeks a commercial or industry partner to further develop and commercialize this innovative platform for free adenosine diphosphate ribose biosensors.