This invention uses advanced optical coatings to enhance fluorescence microscopy by combining two critical functions—filtering and splitting light—into a single, compact component. Streamlining these processes enables the precise isolation of light wavelengths while efficiently splitting them into multiple paths for imaging or analysis. It can be applied in various settings, from live-cell imaging to advanced microscopy techniques like super-resolution imaging and ratiometric sensing. By integrating these functions into one element, this technology enhances microscopy performance in a compact and efficient optical system for use in scientific research and medical diagnostics. Background: Fluorescence microscopy is a powerful tool used in scientific research and medical diagnostics to study biological samples at a cellular level. However, current systems rely on separate filters and beam splitters to isolate and analyze light emissions from different fluorophores. This approach increases the size and complexity of the equipment, creates alignment challenges, and can reduce system efficiency due to stray light and suboptimal performance. This new technology addresses these issues by integrating the functions of filtering and beam splitting into a single compact component. Unlike traditional systems, it uses advanced optical coatings to precisely manage light wavelengths while minimizing stray light. This not only simplifies the design and reduces equipment size but also improves overall performance. The result is a more efficient, streamlined solution that enhances imaging accuracy and usability for a range of applications, from live-cell imaging to super-resolution microscopy. Applications:
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