Enhanced Stability of Proteins Immobilized on Carbon Nanotubes

RPI ID:
2005-030-201

Innovation Summary:
This invention leverages the curvature of nanoparticles, such as carbon nanotubes, to enhance the stability and activity of proteins immobilized on their surfaces. Proteins bound to nanoscale curved surfaces retain biological activity even under harsh conditions, such as high temperature, organic solvents, or extreme pH. This stabilization occurs because the nanoscale curvature suppresses protein-protein aggregation. The compositions can be used in biosensors, drug delivery, and industrial biocatalysis. They expand the potential of enzymes and biomolecules to function in environments previously unsuitable for protein-based applications. The technology represents a major advance in bio-nanotechnology, combining the catalytic efficiency of enzymes with the durability of nanomaterials. It also enables the design of new hybrid materials that integrate biology with nanotechnology for advanced applications.

Challenges / Opportunities:
Proteins are often unstable outside physiological conditions, limiting their application in diagnostics, therapeutics, and industrial catalysis. Conventional immobilization strategies can impair activity or fail to provide protection in hostile environments. This invention addresses these challenges by showing that nanoparticle curvature enhances functional stability. The opportunity lies in developing robust protein-based systems for environmental monitoring, green chemistry, and therapeutic delivery. It also presents an opportunity to design multi-functional nanomaterials that integrate sensing, catalysis, and therapeutic functions in one platform.

Key Benefits / Advantages:
✔ Enhances protein stability in harsh environments
✔ Retains enzymatic activity under stress
✔ Compatible with diverse nanomaterials
✔ Expands enzyme applications in industry

Applications:
• Industrial biocatalysis
• Biosensors
• Protein therapeutics
• Drug delivery systems

Keywords:
Nanoparticles, protein stability, biocatalysis, biosensors, drug delivery

Intellectual Property:
US Patent No. 9,360,475 B2