Dual-Functional Tetrazine Reagents for Bis-Thiol Bioconjugation and Functionalization

This method is for a novel dual-functional tetrazine reagent with application in Cys-specific bioconjugation and functionalization of target proteins. This class of compounds can be utilized for specific bis-thiol targeting. 

Background: 
Bioconjugates are molecules with altered chemical structures via substrates or reagents that allows for biomolecule linkage. The most common types of bioconjugation include coupling of a small molecule (such as biotin or a fluorescent dye) to a protein, including arguably the most common type, Antibody Drug Conjugates. These reactions encounter limitations within the ability to harness efficient methods for incorporation of the tetrazine moiety into the desired biotargets. 

Applications: 

• Bioconjugation and functionalization of target proteins in bi-thiols
• Bioorthothogonal chemistry
• Biomedical research

Advantages: 

• Efficient incorporation of tetrazine moiety for structural diversity
• Well-defined peptide and protein conjugates
• Mild reaction conditions
• Operational simplicity within reaction elements