PAGE TITLE
Overview
PAGE SUMMARY
An innovative method for the precise detection and measurement of individual telomere lengths that can be correlated to specific chromosomes.
This technology provides a method for detecting the length of individual telomeres in genomic DNA using a unique combination of guide RNA, Cas9 nickase, polymerase, and fluorescently labeled nucleotide. The fluorescently labeled motif sequences are used as a barcode to identify the chromosome. This technology can be deployed to detect and map chromosomal haplotypes and previously unknown regions of the human genome.
ADVANTAGES
TITLE:Key Advantages
Simultaneously measures individual telomeres in a single reaction
Identifies corresponding chromosome arms
Capable of identifying chromosomes with no detectable telomere repeats and previously unknown regions of the genome
Uses a smaller amount of DNA compared to other methods
Capable of identifying chromosomal haplotypes
Problem Solved
TITLE:Problems Solved
Overcomes the limitation of traditional methods that only provide mean telomere length information
Solves the problem of existing methods being unable to measure telomeres in senescent cells
Addresses the challenge of measuring critically short telomeres, which are critical for chromosome stability and cell viability
APPLICATIONS
TITLE: Market Applications
Useful in aging research for understanding telomere lengths in healthy humans and their role in diseases such as cancer
Helpful in studying and understanding individual haplotypes and the human genome
Telomere biology research
Biomedical research involving DNA damage and repair
Drug discovery and development
Cancer diagnostics and therapy
IP STATUS
Intellectual Property and Development Status
US Patent Issued - #US11761028B2 - Methods of specifically labeling nucleic acids using CRISPR/Cas9
PUBLICATIONS
References
Pubinfo should be the citation for your publication. Publink is the full url linking to the publication online or a pdf.
Genes 2023: Single-Molecule Telomere Assay via Optical Mapping (SMTA-OM) Can Potentially Define the ALT Positivity of Cancer
BMC Genomics 2020: Single-molecule analysis of subtelomeres and telomeres in Alternative Lengthening of Telomeres (ALT) cells
Genome Research 2017: High-throughput single-molecule telomere characterization
Commercialization Opportunities
This invention is part of a larger portfolio of technologies available for licensing and commercialization: TOOLS FOR GENOMIC APPLICATIONS POWERED BY CRISPR/CAS9 BASED DNA LABELING
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Contact Information
For Intellectual Property and Licensing inquiries
Tanvi Muni, PhD
Licensing Manager,
Drexel Applied Innovation
Office of Research and Innovation
3250 Chestnut Street, Ste. 3010 Philadelphia, PA 19104
Phone:267-359-5640
Email:tanvi.muni@drexel.edu
Inventor information
Ming Xiao, PhD
Professor School of Biomedical Engineering, Science and Health Systems
Phone:215-895-2690
Email:mx44@drexel.edu
Inventor Webpage