The HEK293T/T7 cell line is a novel development in virology research, particularly for studying human noroviruses. This cell line expresses the T7 RNA polymerase, a key enzyme used in reverse genetics systems. Unlike existing technologies, the HEK293T/T7 cell line offers the unique advantage of being able to produce functional T7 RNA polymerase, which is essential for driving transcription from T7 promoters. This capability opens up new possibilities for studying human noroviruses, which cannot be propagated in cell culture, and may facilitate the development of a reverse genetics system for these viruses. Overall, the HEK293T/T7 cell line represents a significant advancement in virology research and offers a valuable tool for researchers studying virus-host interactions and developing therapeutics or vaccines.