(a) Method implemented for intradermal delivery of DNA/RNA using coated microneedle array followed by electroporation, targeting the region between the epidermis and dermis. Blue spots around the microneedles indicated the spray coating dispersion after insertion. (b) Image of 4 x 4 microneedle array coated with 50 mg of plasmid DNA, 25 mg of trehalose, and 5ug of PS-b-PAA.
Invention Summary:
Delivery of DNA vaccines provides a relevant example. DNA vaccines offer major advantages in speed, stability, and scalability, but their adoption has been limited by inefficient intracellular delivery. Conventional injections of naked nucleic acids result in poor uptake, while traditional electroporation, though effective, can be painful, complex, and poorly suited for routine or large‑scale vaccination. This technology enables delivery via electroporation with much lower and safer electric fields.
This Rutegrs invention describes a minimally invasive penetrating microneedle‑microelectrode array designed to deliver biomolecules, such as DNA, RNA, and proteins, directly into skin cells using localized electroporation. Upon insertion into the skin, the biomolecule coating dissolves at the target site. A controlled electrical pulse transiently permeabilizes nearby cell membranes, enabling efficient uptake of the biomolecules.
The device uniquely combines:
Penetrating microelectrodes that create highly localized electric field “hotspots”
Selective electrical insulation to precisely control where electroporation occurs
.
Market Applications:
Intradermal delivery of biomolecules such as DNA, RNA, protein
Targets dendritic‑cell‑rich epidermal tissue for enhanced immune response
Localized gene electrotransfer for therapeutic gene expression
Advantages:
Non-viral delivery of DNA and RNA
Lower voltages and smaller electrode geometry limit skin irritation, edema, and scarring
Compatible with DNA, RNA, proteins, and other biomolecules
Publications:
• Park et. Al., Microneedle arrays coated with middle east respiratory syndrome coronavirus DNA via electrospray deposition. Soft Matter, 2025. https://pubs.rsc.org/en/content/articlelanding/2025/sm/d4sm01322k
Intellectual Property & Development Status: US Patent 12,508,423. Available for licensing and/or research collaboration. For any business development and other collaborative partnerships, contact: marketingbd@research.rutgers.edu