Micro-Ovary: A Self-Assembled Ovary at Microscale Resolution

SHORT DESCRIPTION

An innovative three-dimensional micro-ovary model that recapitulates native ovarian physiology in vitro to enable high-fidelity drug screening and reproductive applications.

INVENTORS
  • Francesca Duncan*
  • Aubrey Converse 
  • Lauren Haky
* Principal Investigator

NU Tech ID:  NU 2025-292

IP STATUS

Pending

DEVELOPMENT STAGE

TRL-4 Prototype Validated in Lab: Self-assembly and follicle encapsulation have been demonstrated in a laboratory setting.

 

BACKGROUND

The ovary is a complex organ composed of follicles developing within a dynamic stromal microenvironment that plays an essential role in regulating follicle growth, maturation, and overall ovarian function. Despite the importance of stromal–follicular interactions, existing ovarian culture systems often fail to fully integrate these subcompartments, limiting their ability to accurately model native ovarian physiology. Prior studies have demonstrated that isolated follicles co-cultured with ovarian stromal feeder cells exhibit enhanced growth, underscoring the critical role of the stromal environment in supporting follicle development. More physiologically relevant ovarian models are therefore needed to better capture the cellular interactions that drive ovarian biology.

ABSTRACT

Northwestern researchers have developed the micro-ovary, a three-dimensional, self-assembled ovarian model that mimics native in vivo physiology on a microscale. It integrates an intact follicle with an engineered stromal microenvironment, enabling dynamic physical, mechanical, and paracrine interactions. Robust validation in laboratory settings using both murine and rhesus macaque cells confirms its capability to encapsulate and support follicle growth. This platform offers significant potential for studies in ovarian biology, fertility treatment development, and reproductive medicine.

APPLICATIONS

  • Drug Screening: In vitro platform for testing drug efficacy and toxicity.
  • Fertility Preservation: Enables ex vivo ovarian tissue support and follicle maturation to preserve reproductive potential.
  • Assisted Reproduction: Aids in the maturation of ovarian follicles and oocytes for improved egg quality.
  • Disease Modeling: Facilitates research into ovarian dysfunction and related disorders.

ADVANTAGES

  • Mimics native ovarian architecture: Integrates follicular and stromal compartments to reflect in vivo conditions.
  • Enhances physiological relevance: Maintains cellular heterogeneity and proper compartmental organization.
  • Rapid self-assembly: Enables quick formation of a robust, integrated ovarian model.
  • Reproducible and scalable: Offers a reliable platform for various in vitro applications.

 

 

 

Patent Information: