PAGE TITLE
Overview
PAGE SUMMARY
A new genome analysis technique that uses guide RNA and Cas9 nickase to detect target nucleic acid sequences using fluorescent labeling.
The technology involves a method for sequence-specific labeling that can target repetitive regions in the genome. This method contacts genomic DNA with a guide RNA and Cas9 nickase to produce a single-strand break (nick) adjacent to the target sequence. A fluorescently labeled nucleotide is incorporated into the nicked DNA, which is then used to detect the target nucleic acid sequence. This technique can be used for genomic research, clinical diagnostics, identification of structural variants, repetitive region mapping, etc.
ADVANTAGES
TITLE:Key Advantages
Allows for precise mapping of long-range de novo assembly contiguity and validation
Can target repetitive regions often lacking in restriction site motif
Capable of detecting specific structural variations, providing accurate breaking points
Offers a potential solution to sequence mis-assembly in complex, duplicated, and repetitive regions
Improved accuracy in detecting target nucleic acid sequence
Creates a barcode of a portion of the genomic DNA
Problem Solved
TITLE:Problems Solved
Inadequate long-range de novo assembly contiguity
Sequence mis-assembly in complex segmentally duplicated and repetitive regions
Detection and delineation of structural variations
Overcomes the limitation of being unable to target repetitive regions
APPLICATIONS
TITLE: Market Applications
Clinical diagnostics for detecting and typing structural variations
Development of new genome editing tools and techniques
Genomic research and analysis
Clinical Diagnostics
IP STATUS
Intellectual Property and Development Status
US Patent Issued #US10640810B2 - Methods of specifically labeling nucleic acids using CRISPR/Cas - Expires 2037
PUBLICATIONS
References
Pubinfo should be the citation for your publication. Publink is the full url linking to the publication online or a pdf.
Nature Scientific Reports 2024: A long-read sequencing strategy with overlapping linkers on adjacent fragments (OLAF-Seq) for targeted resequencing and enrichment
Nucleic Acids Research 2015: CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double strand DNA for whole genome mapping and structural variation analysis
Commercialization Opportunities
This invention is part of a larger portfolio of technologies available for licensing and commercialization TOOLS FOR GENOMIC APPLICATIONS POWERED BY CRISPR/CAS9 BASED DNA LABELING
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Contact Information
For Intellectual Property and Licensing inquiries
Tanvi Muni, PhD
Licensing Manager,
Drexel Applied Innovation
Office of Research and Innovation
3250 Chestnut Street, Ste. 3010 Philadelphia, PA 19104
Phone:267-359-5640
Email:tanvi.muni@drexel.edu
Inventor information
Ming Xiao, PhD
Professor School of Biomedical Engineering, Science and Health Systems
Phone:215-895-2690
Email:mx44@drexel.edu
Inventor Webpage