Current pre-clinical drug discovery incompletely recapitulates the complexity and heterogeneity of human disease and use of decellularized tissues and organ scaffolds to provide better model systems has drawn great interest, especially in complex organs such as the lung. Acellular lung tissue scaffolds from both normal and diseased human lung tissues provide a more biologically relevant three-dimensional structure that preserves disease phenotypes and bridges the gap between cell culture, animal models and human disease. The Weiss lab, experts in lung regeneration and repair, have developed a portfolio of novel techniques and components to create a high throughput decellularization kit for the lung. The DC Kit contains optimized decellularization reagents, detection systems for residual detergents, and a novel alginate coating technology to maintain the 3D structure and mechanical integrity of the decellularized tissue.